Automated processing of zebrafish imaging data - a survey

R. Mikut, T. Dickmeis, W. Driever, P. Geurts, F. Hamprecht, B. Kausler, M. Ledesma-Carbayo, R. Marée, K. Mikula, P. Pantazis, Olaf Ronneberger, A. Santos, R. Stotzka, U. Strähle, N. Peyriéras
Zebrafish, 10(3): 401--421, Aug 2013
Abstract: Due to the relative transparency of its embryos and larvae, the zebrafish is an ideal model organism for bioimaging approaches in vertebrates. Novel microscope technologies allow the imaging of developmental processes in unprecedented detail, and enable the use of complex image-based read-outs for high-throughput/high-content screening. Such applications can easily generate Terabytes of image data, the handling and analysis of which becomes a major bottleneck in extracting the targeted information. Here, we describe the current state-of-the-art in computational image analysis in the zebrafish system. We discuss challenges encountered when handling high-content image data, especially regarding data quality, annotation and storage. We survey methods for preprocessing image data for further analysis, and describe selected examples of automated image analysis, including the tracking of cells during embryogenesis, heartbeat detection, identification of dead embryos, recognition of tissues and anatomical landmarks, and quantification of behavioral patterns of adult fish. We review recent examples for applications using such methods, such as the comprehensive analysis of cell lineages during early development, the generation of a 3D brain atlas of zebrafish larvae, and high-throughput drug screens based on movement patterns. Finally, we identify future challenges for the zebrafish image analysis community, notably those concerning the compatibility of algorithms and data formats for the assembly of modular analysis pipelines.
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  author       = "R. Mikut and T. Dickmeis and W. Driever and P. Geurts and F. Hamprecht and B. X. Kausler and M. J. Ledesma-Carbayo and R. Mar{\'e}e and K. Mikula and P. Pantazis and O. Ronneberger and A. Santos and R. Stotzka and U. Str{\"a}hle and N. Peyri{\'e}ras",
  title        = "Automated processing of zebrafish imaging data - a survey",
  journal      = "Zebrafish",
  number       = "3",
  volume       = "10",
  pages        = "401--421",
  month        = "Aug",
  year         = "2013",
  url          = "http://lmb.informatik.uni-freiburg.de/Publications/2013/Ron13"

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