Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation

M. Jordão, R. Sankowski, S. Brendecke, S. Sagar, G. Locatelli, Y. Tai, T. Tay, E. Schramm, S. Armbruster, N. Hagemeyer, O. Groß, Dominic Mai, Özgün Çiçek, Thorsten Falk, M. Kerschensteiner, D. Grün, M. Prinz
Science, 363(6425): eaat7554, Jan 2019

The innate immune cell compartment is highly diverse in the healthy central nervous system (CNS), including parenchymal and non-parenchymal macrophages. However, this complexity is increased in inflammatory settings by the recruitment of circulating myeloid cells. It is unclear which disease-specific myeloid subsets exist and what their transcriptional profiles and dynamics during CNS pathology are. Combining deep single-cell transcriptome analysis, fate mapping, in vivo imaging, clonal analysis, and transgenic mouse lines, we comprehensively characterized unappreciated myeloid subsets in several CNS compartments during neuroinflammation. During inflammation, CNS macrophage subsets undergo self-renewal, and random proliferation shifts toward clonal expansion. Last, functional studies demonstrated that endogenous CNS tissue macrophages are redundant for antigen presentation. Our results highlight myeloid cell diversity and provide insights into the brain’s innate immune system.

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  author       = "M. J. C. Jordão and R. Sankowski and S. M. Brendecke and Sagar and G. Locatelli and Y. Tai and T. L. Tay and E. Schramm and S. Armbruster and N. Hagemeyer and O. Groß and D. Mai and {\"O}. {\c{C}}i{\c{c}}ek and T. Falk and M. Kerschensteiner and D. Gr{\"u}n and M. Prinz",
  title        = "Single-cell profiling identifies myeloid cell subsets with distinct fates during neuroinflammation",
  journal      = "Science",
  number       = "6425",
  volume       = "363",
  pages        = "eaat7554",
  month        = "Jan",
  year         = "2019",
  url          = "http://lmb.informatik.uni-freiburg.de/Publications/2019/MCF19"

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